Figure 4.
Analysis of the antibody response to B. burgdorferi B31-5A4 wildtype or ospC transgenic strains in mice. ELISAs were conducted using serum harvested from mice 4 weeks after needle inoculation (infecting strain indicated along the X axis). The sera were serially diluted, screened against immobilized whole B. burgdorferi B31-5A4 wild type cells and titers calculated for each mouse at 1/3 ODmax (indicated by triangles). Geometric means are indicated by horizontal lines. In panel B, immunoblots of whole cell lysates of B. burgdorferi B31-5A4 wild type were screened with serum collected 4 weeks after inoculation with wild type or transgenic B. burgdorferi B31strains (as indicated above each lane).