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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Mol Microbiol. 2010 Feb 28;76(2):393–408. doi: 10.1111/j.1365-2958.2010.07103.x

Figure 6.

Figure 6

Circular dichroism and blue-native PAGE analyses of recombinant OspC site-directed substitution mutants. Recombinant protein was generated for wild type-type A OspC and each site-directed mutant as detailed in the text. The CD spectra are presented in the figure. The estimated alpha helical content of each protein is provided in the upper right inset. The results of blue native PAGE analyses, conducted to assess the ability of each recombinant protein to dimerize, are shown in the lower right inset. All methods were as described in the text.