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Plasminogen binding by recombinant OspC proteins. Human plasminogen, immobilized in ELISA plate wells, was screened with His-tagged wild type and mutant recombinant OspC proteins (indicated in the figure). Binding was detected with mouse-anti-His tag monoclonal antibody and peroxidase-conjugated goat-anti-mouse IgG. All methods are detailed in the text.
