Table 1.
Oligonucleotides used in this study
| Primer | Description | Sequence |
|---|---|---|
| pCAEV1 Upstream (+) | pCAEV1 vector construction | CAGAATGAGTTACTTCTGGATGG |
| pCAEV1 OspC 5′ (−) BspEI/MluI | PCAEV1 vector | GTACGCGTTTTTCCGGAATTATTACAAGATATAA |
| pCAEV1 OspC 3′ (+) BspEI | PCAEV1 vector | TCCGGATCAATATTATAAGATTAATTTGTTTTAAA |
| pCAEV1 BBB 21 3′ (−) AatII | PCAEV1 vector | GACGTCATCTCACATAAAACCAAAGAAACTAC |
| Spec/Strep (+) SalI | PCAEV1 vector | GTCGACTAATACCCGAGCTTCAAGGA |
| Spec/Strep (−) AatII | PCAEV1 vector | GACGTCATTATTTGCCGACTACCTTGG |
| pCAEV1 Downstream (+) SalI | PCAEV1 vector | GTCGACTTTAAAAAGTTGTTAAATAGACTTAACT |
| pCAEV1 Downstream (−) MluI | PCAEV1 vector | ACGCGTGGATATATGCAATCTTTAGTCCAG |
| Insert type A (+) BspEI | Amplification of insert | TCCGGAAAAGATGGGAATACATCTGCA |
| Insert type A (−) BspEI | Amplification of insert | TCCGGATTAAGGTTTTTTTGGACTTTCTG |
| Upstream confirm (+) | Transformation confirmation | CCTACGTTGTGATGAGACTTGATTT |
| Spec/strep 3′ (+) | Transformation confirmation | GGCGAGATCACCAAGGTAGTC |
| Insert sequencing (+) | OspC sequence | TAAAAAGGAGGCACAAATTAATG |
| Deletion upstream (+) | Deletion vector construction | GCAACAATCCAGTGTTTACAAAAACG |
| Deletion upstream (−) | Deletion vector construction | ACCGGTTCTGACGTCTAATTTGTGCCTCCTTTTT |
| Deletion downstream (+) | Deletion vector construction | GACGTCGTTGTGGCAGAAAGTCCAAAAAAACC |
| Deletion dow nstream (−) | Deletion vector construction | ACCGGTGCTGTTTAACGATTTATTTGATACTTTG |
| OspC (+) LIC | Amplification of OspC for | GACGACGACAAGATTAATAATTCAGGGAAAGAT |
| OspC (−) LIC | Amplification of OspC for | GAGGAGAAGCCCGGTTTAAGGTTTTTTTGGACT |
| OspC E61Q/E63Q (+) | Mutagenic primer | GTGAAACAGGTTCAAGCGTTG |
| OspC E61Q/E63Q (−) | Mutagenic primer | CAACGCTTGAACCTGTTTCAC |
| OspC K60Y (+) | Mutagenic primer | CAACGCTTCAACCTCATACAC |
| OspC K60Y (−) | Mutagenic primer | GTGTATGAGGTTGAAGCGTTG |
| OspC E61Q (+) | Mutagenic primer | GTGAAACAGGTTGAAGCGTTG |
| OspC E61Q (−) | Mutagenic primer | CAACGCTTCAACCTGTTTCAC |
| OspC E63Q (+) | Mutagenic primer | GTGAAAGAGGTTCAAGCGTTG |
| OspC E63Q (−) | Mutagenic primer | CAACGCTTGAACCTCTTTCAC |
| FlaB q-PCR primer (+) | q-PCR primer | CAGGTAACGGCACATATTCAGATGC |
| FlaB q-PCR primer (−) | q-PCR primer | CTTGGTTTGCTCCAACATGAACTC |
| Nid1 q-PCR primer (+) | q-PCR primer | CCAGCCACAGAATACCATCC |
| Nid1 q-PCR primer (−) | q-PCR primer | GGACATACTCTGCTGCCATC |