Figure 3.

Astrocytic Ca²⁺ signals in vivo in mice 3 d after SE are suppressed by the mGluR5 antagonist MPEP. A, Fluo-4-labeled astrocytes before (left) and after (right) the mouse was coinjected with MPEP and rhodamine-dextran through the tail vein. B, Calcium signals of a cell pair were inhibited after MPEP injection. C, Calcium signals before and after acute administration of MPEP (1 mg/kg tail vein injection), depicted as the ΔF/F₀ integral. Also shown is the inhibition by four daily intraperitoneal administrations of MPEP (20 mg/kg). The NR2B NMDA receptor antagonist ifenprodil (20 μm) does not affect astrocytic Ca²⁺ signals. *p < 0.002 (n = 4 animals in each group except ifenprodil, n = 3). Error bars indicate SEM.