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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: Nature. 2009 Aug 9;460(7259):1132–1135. doi: 10.1038/nature08235

Figure 1. iPS cell generation from p53-null MEF by four or three reprogramming factors.

Figure 1

a. iPS cells were generated from Nanog-GFP reporter MEF, which were either p53 wild-type (+/+), heterozygous (+/-), or homozygous (-/-), by the three reprogramming factors (Oct3/4, Sox2, Klf4). After retroviral transduction, 5000 live cells were collected by a flow cytometer. GFP-positive colonies were counted 28 days after the transduction. *, p<0.05 compared to wild-type (n=4).

b. iPS cells were generated by the three factors from single sorted cells in wells of 96-well plates. GFP-positive colonies were counted 28 days after the transduction. **, p<0.01 compared to wild-type (n=4).

c. iPS cells were generated by the four factors, including c-Myc, from single sorted cells in wells of 96-well plates. GFP-positive colonies were counted 21 days after the transduction. *, p<0.05 compared to wild-type (n=4).

d. Retroviruses expressing either the dominant negative p53 mutant (P275S) or wild-type were co-transduced with the three factors into Nanog-GFP, p53 heterozygous MEFs. After retroviral transduction, 5000 cells were collected and GFP-positive colonies were counted 28 days after the transduction. As a control, retrovirus for a red fluorescent protein (DsRed) was transduced. *, p<0.05 compared to DsRed control (n=3).

e. Retroviruses expressing either the wild-type or mutant p53 were co-transduced with the three factors into Nanog-GFP, p53-null MEFs. After retroviral transduction, 5000 live cells were collected and GFP-positive colonies were counted 28 days after the transduction. *, p<0.05 compared to DsRed control (n=3).