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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: Nature. 2009 Aug 9;460(7259):1132–1135. doi: 10.1038/nature08235

Figure 2. T-lymphocyte-derived iPS cells.

Figure 2

a. Protocol for iPS cell generation from mouse T-lymphocytes. TCM; T cell medium, ESM; ES cell medium.

b. A phase contrast image, Nanog-GFP expression, alkaline phosphatase staining, and SSEA1 staining of T cell-derived iPS cells (clone 408E2). Bars indicate 100 μm.

c. Expression of marker genes was examined by RT-PCR in T cell-derived iPS cells (clones 408E -2, -7 and -8), RF8 ES cells, T cells, Spleen, Fbx15-selected iPS cells from p53 wild-type MEF (clone 7B3), and Nanog-selected iPS cells from p53 wild-type MEF (clone 38D2)

d. A chimera mouse derived from clone 408E2. iPS cells were microinjected into blastocysts from ICR mice.

e. The V-(D)-J DNA rearrangement of the Tcrβ gene was confirmed by genomic PCR in iPS cells and a chimeric mouse. GL indicates the germline band.