Table 1. Relative CMM2 expression levels.
Construct boundaries | Expression level | Purification | Crystals obtained | Maximum resolution (Å) |
---|---|---|---|---|
1675–2001 | − | |||
1675–1823 | ++ | Yes | ||
1692–1805 | ++ | Yes | ||
1692–1817 | ++ | |||
1692–1823 | ++ | Yes | ||
1692–2001 | ||||
1699–1805 | + | Yes | ||
1699–1813 | +++ | Yes | ||
1699–1814 | +++ | Yes | Yes | 3.6–3.8 |
1699–1817 | +++ | |||
1699–1823 | ++ | |||
1700–1813 | +++ | |||
1700–1814 | +++ | Yes | Yes | 3.2–3.5 |
1715–1805 | + |