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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: J Immunol. 2010 May 28;185(1):424–432. doi: 10.4049/jimmunol.0903291

FIGURE 6.

FIGURE 6

RIG-I, RIPLET, and TRIM25 expression in uninfected and NDV-infected 293T cells and human monocyte-derived DCs. Western blot showing the level of protein expression for RIG-I, two isoforms of RIPLET (gene RNF135) and TRIM25 (gene TRIM25) at 0, 6, and 12 h after NDV infection in 293T cells and human DCs. A, Time course after NDV infection for RIG-I, RIPLET, and TRIM25 expression in transfected 293T cells. Normalized to tubulin. B, Same time course for donor 1 DCs. Arrowheads denote TRIM25 and the two isoforms of RIPLET. C, Combined assay of transfected 293T cells and DCs from a second donor with (+) or without (−) NDV infection (10 h). C7, RIG-I Cys7 transfection; Ctr, no transfection; R7, RIG-I Arg7 transfection.