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. Author manuscript; available in PMC: 2011 Jun 18.
Published in final edited form as: J Mol Biol. 2010 Apr 29;399(4):576–595. doi: 10.1016/j.jmb.2010.04.038

Figure 3. Biochemical control experiments confirm the existence of three intermediate states during tRNA selection on the ribosome.

Figure 3

(A) Contour plots and histograms of FRET trajectories obtained during the delivery of Cy5-labeled ternary complex to ribosome complexes containing Cy3-labeled P-site tRNAfMet. Histograms were fit to the sum of three Gaussian distributions with means reflecting the AC state (~0.54), the GA state (~0.32), and the CR state (~ 0.21). (B) Contour plots and histograms obtained during the delivery of Cy5-labeled ternary complex to ribosome complexes containing Cy3-labeled L11. Histograms were fit to the sum of two Gaussian distributions with means reflecting the AC state (~0.46), and the GA and CR states (~0.73). (Left panels) ternary complex delivery to the near-cognate (CUU) complex. (center-left panels) ternary complex delivery to the cognate (UUC) complex in the presence of 1mM GDPNP, (center-right panels) ternary complex delivery to the cognate (UUC) complex in the presence of 20μM kirromycin (Right panels) ternary complex delivery to the cognate (UUC) complex.