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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Lab Invest. 2009 Oct 19;89(12):1410–1422. doi: 10.1038/labinvest.2009.88

Figure 6.

Figure 6

K19-positive MSCs in the peripheral blood of mice with chronic H. felis infection. (a) Expression of K19 mRNA in freshly isolated mononuclear cell fraction in bone marrow (BM) or peripheral blood (PB) of mice with or without H. felis-associated chronic gastritis. (b) Attached cell fraction 14 days after seeding of peripheral blood from mice with chronic H. felis infection. (c) Adipocyte and osteocyte differentiation of MSC clones established from peripheral blood. PBMSCs were incubated with adipocyte or osteocyte differentiation medium for 14 days and cells were stained with Oil red-O or Alizarin Red, respectively. (d) GFP-positive cells in MSC culture established from peripheral blood of C57/BL6 mice that received bone marrow transplantation from chicken β-actin EGFP donor mice and subsequent chronic H. felis infection. (e) Expression of gastric epithelial phenotype markers in peripheral blood-derived MSC clones after treatment with gastric tissue extract (GL). Cells were incubated with gastric tissue extract for 5 days and the mRNA expression of K19, TFF2, Muc5as, Muc6, H/K-ATPase, IF, and CGA were detected by real-time PCR.