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. Author manuscript; available in PMC: 2010 Aug 6.
Published in final edited form as: Cytometry A. 2009 Mar;75(3):264–270. doi: 10.1002/cyto.a.20670

Figure 3.

Figure 3

Internalization of fluorescent ligand bound FPRL1 and colocalization with arrestin-3 in RBL cells. The cells were all exposed to 5 nm WK(FL)YMVm for 10 min at 37°C before being washed and fixed. A,B: Internalization of the fluorescent WK(FL)YMVm ligand (green) is demonstrated in wild-type RBL cells (A) and FPRL1 expressing cells (B). Nuclei are stained with DAPI (blue). C_E: Colocalization of fluorescent ligand with arrestin-3. RBL cells transiently transfected with RFP-arrestin-3 (D) were allowed to internalize ligand as before (C); merged image in E.