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Replication kinetics of influenza A viruses in MDCK(SH3+) and control MDCK(SH3-) cells. Cells were infected with influenza A viruses at an MOI of 0.001 and incubated with serum-free medium containing 1 ug/ml TPCK-trypsin. The yield of virus in the culture supernatant was titrated every 12 h, from 12 h post-infection, by plaque assay in MDCK cells. The time point where maximal reduction was observed is indicated with asterisk (*).
