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Western blot analysis of phosphorylated Akt level in MDCK cells. MDCK cells with or without heterologous SH3 were infected with PR8, ST1233, ST364, Ph2246, or Qa199 at an MOI of 2 for 8 h. Akt-P (phosphorylated Akt at Serine 473) and Akt-T (total Akt) in cellular lysate were detected using anti-Akt-P and anti-Akt-T antibodies, respectively, and peroxidase-conjugated secondary antibody.
