Table 2.
Variable | d 0 pre-transport | d 2 Arrival in French lairage | D 3 post-12 h lairage | d 4 arrival in Spain | d 6 post-transport | d 8 post-transport | d 10 post-transport | d 34 post-transport |
---|---|---|---|---|---|---|---|---|
White blood cell (WBC) number (1×103 μL) | ||||||||
Control | 9.20 ± 0.57ax | 8.20 ± 0.41abx | 8.60 ± 0.54abx | 7.90 ± 0.75abx | 8.90 ± 0.75abx | 7.81 ± 0.49abx | 7.40 ± 0.44bx | 9.66 ± 0.47bx |
RT | 10.10 ± 0.57ax | 20.90 ± 2.30by | 15.40 ± 1.99by | 23.10 ± 1.43by | 15.00 ± 1.43by | 12.60 ± 1.01by | 18.70 ± 1.74by | 13.10 ± 1.22by |
ULT | 9.00 ± 0.59ax | 16.70 ± 16.0by | 14.0 ± 1.16by | 17.80 ± 1.31by | 15.60 ± 1.31by | 14.10 ± 1.45by | 16.10 ± 1.07by | 11.14 ± 0.95by |
Neutrophil percentage (%) | ||||||||
Control | 36.25 ± 2.70abx | 32.95 ± 2.705bx | 38.22 ± 3.10abx | 33.20 ± 3.00bx | 35.95 ± 2.85bx | 38.10 ± 2.11abx | 42.10 ± 2.42ax | 32.44 ± 3.30bx |
RT | 38.00 ± 2.72abx | 37.16 ± 2.76abx | 39.20 ± 2.97abx | 43.95 ± 3.01bx | 37.16 ± 2.92abx | 42.67 ± 2.21bx | 33.79 ± 2.50axy | 40.68 ± 3.10abx |
ULT | 37.30 ± 2.70abx | 34.35 ± 2.70abx | 37.78 ± 3.10abx | 38.84 ± 3.00abx | 34.55 ± 2.85abx | 37.53 ± 2.25abx | 31.47 ± 2.48aby | 36.06 ± 3.23abx |
Lymphocyte percentage (%) | ||||||||
Control | 62.35 ± 2.86abx | 64.26 ± 2.74abx | 60.11 ± 3.02bx | 65.85 ± 2.92abx | 62.85 ± 2.87abx | 60.40 ± 2.05abx | 57.30 ± 2.47ax | 64.88 ± 14.14abx |
RT | 59.80 ± 2.90abx | 58.63 ± 2.70abx | 59.15 ± 2.92abx | 53.89 ± 3.00bx | 61.37 ± 2.14abx | 54.94 ± 2.53bx | 65.68 ± 3.10axy | 58.42 ± 15.62abx |
ULT | 59.15 ± 2.80abx | 62.05 ± 2.70abx | 59.89 ± 3.01abx | 59.00 ± 2.96abx | 63.25 ± 2.86abx | 60.82 ± 2.20abx | 68.32 ± 2.53aby | 62.82 ± 12.51abx |
Neutrophil:Lymphocyte ratio | ||||||||
Control | 0.81 ± 0.13abx | 0.70 ± 0.08abx | 0.73 ± 0.10abx | 1.00 ± 0.13bx | 0.68 ± 0.09abx | 0.82 ± 0.06abx | 0.59 ± 0.08ax | 0.89 ± 0.13abx |
RT | 0.70 ± 0.13abx | 0.61 ± 0.08abx | 0.79 ± 0.10abx | 0.81 ± 0.13bxy | 0.64 ± 0.08abx | 0.65 ± 0.06by | 0.49 ± 0.07axy | 0.64 ± 0.13abx |
ULT | 0.69 ± 0.13abx | 0.58 ± 0.78abx | 0.70 ± 0.10abx | 0.54 ± 0.13aby | 0.63 ± 0.08abx | 0.67 ± 0.06abxy | 0.80 ± 0.07aby | 0.57 ± 0.14abx |
Haematocrit (HCT) (%) | ||||||||
Control | 39.49 ± 0.57axy | 39.09 ± 0.41abx | 38.32 ± 0.54bx | 36.71 ± 0.66bx | 36.51 ± 0.75bx | 36.54 ± 0.49bx | 35.39 ± 0.44bx | 33.69 ± 0.47bx |
RT | 39.18 ± 0.67ax | 34.41 ± 0.74by | 32.17 ± 1.25by | 32.26 ± 0.56by | 31.36 ± 0.65by | 29.75 ± 0.68by | 29.78 ± 0.63by | 28.09 ± 0.66by |
ULT | 40.23 ± 0.59ay | 33.63 ± 0.90by | 32.06 ± 1.16by | 32.98 ± 1.69by | 32.01 ± 1.31by | 30.97 ± 1.45by | 30.74 ± 1.07by | 28.97 ± 0.95by |
The values are expressed as mean ± s.e. Control = not transported; RT (transported animals that remained on the transporter at the French lairage for 12 h) and ULT animals transported (transported animals that were unloaded at the French lairage for a 12 h rest period) at a stocking density of 0.93 m2 per animal;a,bWithin a row, means not having a common superscript differ significantly (P < 0.05);x,ywithin a column at each sampling time point (day (d)), treatment means differ by P < 0.05. Data were analysed using SAS/STAT (9.1 (SAS Inst. Inc., Cary, NC, USA). The differences between means were tested using the Tukey-Kramer test for multiple comparisons.