Table 4.

Antibodies used in the immunostaining of leukocyte surface markers in beef calves

Specificity	Cell types	Clone	Isotype	Working solution	Source1
CD4	T-helper/inducer cells	CC8	IgG2a	7 μL/mL	Serotec
CD8	T-cytotoxic/suppressor cells	CC63	IgG2a	7 μL/mL	Serotec
WC1	Subset of γδ T cells	CC15	IgG2a	7 μL/mL	Serotec
MHC class II	Antigen presenting cells (B cells, activated T cells)	H42A	IgG2a	7 μL/mL	VMRD
CD62L	L-selectin	BAQ92A	IgG1	14 μL/mL	VMRD
G1	Neutrophils (and eosinophils)	MM20A	IgG1	14 μL/mL	VMRD
CD45	All leukocytes (pan marker)2	CC1	IgG1	3.5 μL/mL	Serotec

¹Serotec (Oxford, UK), VMRD (Pullman, WA, USA).

²Used to differentiate leukocyte populations and exclude debris (gate analysis).