Table 4.
Specificity | Cell types | Clone | Isotype | Working solution | Source1 |
---|---|---|---|---|---|
CD4 | T-helper/inducer cells | CC8 | IgG2a | 7 μL/mL | Serotec |
CD8 | T-cytotoxic/suppressor cells | CC63 | IgG2a | 7 μL/mL | Serotec |
WC1 | Subset of γδ T cells | CC15 | IgG2a | 7 μL/mL | Serotec |
MHC class II | Antigen presenting cells (B cells, activated T cells) | H42A | IgG2a | 7 μL/mL | VMRD |
CD62L | L-selectin | BAQ92A | IgG1 | 14 μL/mL | VMRD |
G1 | Neutrophils (and eosinophils) | MM20A | IgG1 | 14 μL/mL | VMRD |
CD45 | All leukocytes (pan marker)2 | CC1 | IgG1 | 3.5 μL/mL | Serotec |
1Serotec (Oxford, UK), VMRD (Pullman, WA, USA).
2Used to differentiate leukocyte populations and exclude debris (gate analysis).