Figure 1.

Layout of scanning light-sheet microscope. The laser beam power is modulated with an acousto-optic modulator (AOM) and expanded with a 2 to 5×beam expander. The beam is then focused into a sample with an f=150-mm lens. The sample is mounted on a motorized rotary stage, which is itself mounted on a platform whose height is controlled by a motorized linear translation stage. Fast horizontal scanning of the laser beam is performed with a piezomounted tilt mirror to produce a light sheet. Uniform and structured illumination images are acquired during scan fly-forward and fly-back, respectively. Slow vertical light-sheet tracking is ensured with a motorized tilt mirror. The setup is displayed as a top view. The inset in dashed lines is a front view.