Figure 2.

Scanning light-sheet images of an optically cleared whole mouse brain (P14) whose hippocampal pyramidal neurons have been targeted with EGFP via in utero electroporation (Cb: cerebellum, Cx: cortex, Hi: hippocampus, Mid: midbrain, Str: striatum, CA1∕3: area of Ammon’s horn, DG: dentate gyrus). Top panels are images acquired with (a) uniform and (b) structured sheet illumination. (c) is the resultant HiLo processed image. Sparsely labeled neurons stand out as bright fluorescent objects amid lower level autofluorescence. (d), (e), and (f) are zoomed maximum intensity projections (54-μm depth range) of a single cell. Fields of view are 8 mm (top) and 2.3 mm (bottom). Laser power was ∼1 mW. Camera exposure time per image was 250 ms.