Figure 3.

Scanning light-sheet images of the half forebrain region of an optically cleared whole mouse brain of older age P45 (Cx: cortex, Mid: midbrain, Str: striatum). Images are acquired in a dark-field scattering mode. A network of myelinated axon processes in the alveus (Alv) of the hippocampus and striatum is clearly visible. Grayscale panels are average intensity projections (∼100-μm depth range) of (a) uniform and (b) structured illumination images, and (c) the corresponding HiLo images. Field of view is 4.8 mm. Laser power was ∼0.15 mW (no emission filter). Camera exposure time per image was 350 ms.