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Combined fluorescence (green) and dark-field (red) HiLo images of the hippocampus region of a whole thy-1 mouse brain (same sample as in Fig. 3). (a), (b), and (c) are frames acquired from various depths within an image stack. The field of view is 4.8 mm. The laser power was 1 and 0.15 mW for fluorescence and dark-field imaging, respectively. The camera exposure time was 350 ms per frame.
