Fig. 5. CPT activates Akt in a concentration-dependent manner.
Serum-starved (A,B) or non-starved (C,D) Rh30 and DU145 cells were pre-treated with CPT (0–40 µM) for 2 h, and then stimulated with (A,B) or without (C,D) IGF-1 (10 ng/ml) for 1 h. The cell lysates were subjected to Western blot analysis with indicated antibodies. β-Tubulin was used for loading control.