(A) Rh30 cells, grown in 6-well plates and infected with Ad-GFP or Ad-mTOR-RD for 24 h, and treated with or without CPT (10 µM) for 2 h, followed by stimulation with or without IGF-1 (10 ng/ml) for 1 h. The cell lysates were subjected to Western blot analysis with indicated antibodies. β-Tubulin was used for loading control. (B,C) Rh30 cells, grown in 6-well plates and infected with Ad-GFP or Ad-mTOR-RD for 24 h, were treated with CPT (0–20 µM) respectively for 24 h, followed by cell counting using a Beckman Coulter Counter (B), or cell cycle analysis (C). Results are presented as mean ± SE (n = 3). *P < 0.05, difference vs. control.