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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nat Med. 2010 Jul 18;16(8):915–920. doi: 10.1038/nm.2182

Fig. 4. Long-lived immune responses.

Fig. 4

Mice immunized with influenza virus by microneedle or IM route of delivery were challenged with live homologous virus 90 days after immunization; Bronchoalveolar lavage fluid (BALF) and sera were collected at four days after challenge. (a) Lung virus titers determined by plaque assay. (b) Lung IgA titers determined by quantitative ELISA. (c) Lung IgG titers and their isotypes. (d) Serum IgG titers and their isotypes. (e) Serum HAI titers after infection determined as geometric mean titers with 95% confidence intervals. (f) Serum IgG2a/IgG1 ratio on days 14 and 28 after immunization and on day 4 after challenge. (g) Anti-influenza IgG antigen-secreting cells (ASC) from splenocytes re-stimulated with inactivated influenza virus. (h) Lung anti-influenza IgG ASC cells. Groups are as described in Fig. 3.