Table 2.
Mean±SEM of passive tension (PT) and active tension (AT) in WT and N2B KO myocardium (n=5 per genotype) before and after PKA treatment.
SL (µm) |
PT (mN/mm2) |
Max AT (mN/mm2) |
nH | pCa50 | ΔpCa50 | |
---|---|---|---|---|---|---|
WT | 1.95 | ------- | 39±2 | 2.9±0.05 | 5.79±0.01 | ------ |
WT | 2.3 | 6.6±1.5 | 50±3 | 2.2±0.11 | 5.92±0.02 | 0.13±0.01 |
PKA- WT | 1.95 | ------- | 41±3 | 2.7±0.06# | 5.70±0.02## | ------ |
PKA- WT | 2.3 | 6.0±1.3# | 52±4 | 2.0±0.07# | 5.89±0.02## | 0.19±0.02# |
KO | 1.95 | ------- | 42±3 | 2.7±0.06 | 5.81±0.02 | ------ |
KO | 2.3 | 16.5±1.1*** | 48±3 | 2.7±0.02** | 6.01±0.01** | 0.21±0.02** |
PKA- KO | 1.95 | ------- | 42±3 | 2.6±0.09 | 5.72±0.02## | ------ |
PKA- KO | 2.3 | 15.0±1.1***/### | 48±3 | 1.5±0.04***/# | 5.99±0.01**/## | 0.27±0.02**/## |
Asterisks: comparison between KO and corresponding WT data (t-test);
Number sign: comparison between before and after PKA incubation;