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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Clin Immunol. 2010 May 15;136(3):400–408. doi: 10.1016/j.clim.2010.04.016

Figure 1. Vβ3+ cells that expand after agonistic antibody stimulation have a predominantly naïve phenotype.

Figure 1

Figure 1

A. PBMCs from a healthy control donor were depleted of CD45RO+ or CD45RA+ cells by magnetic bead depletion. Remaining cells were greater than 97% depleted of the respective subsets. CD4+ cells were labeled with CFSE tracking dye and incubated with anti-Vβ3 antibodies for 4 d. CFSE concentration is on the X-axis and Vβ3 positivity is on the Y-axis. Virtually all the proliferative activity is accounted for by the CD45RO-depleted cells: CD45RA-depleted cells show virtually no proliferative response to Vβ3 stimulation. B. Entry into cell cycle in response to Vβ3 and Vβ5 stimulation, as assessed by Ki67 expression, is also largely restricted to cells with a naïve phenotype. Ki67 expression is plotted along the X-axis. CD45RO-depleted cells (two middle columns) are virtually indistinguishable from unfractionated PBMCs (two leftmost columns), showing robust cell cycle progression after 2-day Vβ3 and Vβ5 stimulation, whereas RA-depleted cells (two rightmost columns) show minimal response.