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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Clin Immunol. 2010 May 23;136(3):338–347. doi: 10.1016/j.clim.2010.04.013

Fig.4.

Fig.4

Cytolytic function of the DMF5 TCR transduced T cells show cytotoxicity against melanoma cells. (A). Staining for surface exposure CD107a by CD4+CD25− (upper) and CD8+ (lower) T cells, when stimulated by A375, MART-127–35 peptide pulsed A375 cells (A375+M1) and the HLA-A2/MART-1 positive line, PT-M. The filled area represents isotype; black line shows interaction with the targets alone; red line shows the interaction with the targets pulsed by the control peptide MAGE-3271–279; and blue line shows the interaction with the targets pulsed with the MART-127–35 peptide. (B). Cytotoxicity by the DMF5 TCR transduced CD4+CD25− (i) and CD8+ (ii) T cells in 4hr 51Cr release assay (PT-M & M202: naturally MART-1 expressing human melanoma lines, A375: HLAA2+/MART-1neg. line; and A375+M1: MART-127–35 peptide pulsed A375 cells). Representative of three separate experiments is shown.