Fig.5.
Amplification of the CD8+ CTL expansion by the DMF5 TCR transduced CD4+CD25− T cells. (A). A representative experiment is shown where untransduced CD8 T cells were incubated with autologous mature DC pulsed with MART-1 peptide in the presence or absence of CD4F5 T cells without any exogenous cytokine. A representative of four separate experiments is shown. (B). Composite data from similar co-culture experiments with TCR transduced CD4+CD25– T cells and untransduced CD8+ T cells from 4 different donors. Fold increase of MART-127–35 specific population (mean ± SEM) was determined by counting the MART-1 tetramer positive cells in flowcytometry with CD8 gating. Of note, no expansion was observed with the mock-transduced CD4+ T cells or with the TCR-transduced CD4+CD25− T cells when set up against the MAGE-3271–279 control peptide, M3. Results from 4 experiments with 4 different donors are shown.