Figure 6.
Sensitivity of the fluorescent CAMB system for adenosine detection. (a) Time-dependent fluorescence enhancement of the sensing system in the presence of different concentrations of adenosine with 10 mM Mg2+ as a cofactor. The concentration of substrate and the aptamer-appended DNAzyme was 300 nM and 200 nM, respectively, and the buffer solution contained 25 mM HEPES (pH 7.2) and 100 mM NaCl. (b) Calibration curve of the fluorescent CAMB sensor for adenosine. Inset shows the linear responses of sensing system to adenosine at low concentration.