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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nat Med. 2010 Jul 18;16(8):872–879. doi: 10.1038/nm.2181

Figure 2.

Figure 2

CIB1 is necessary for pathological but not physiological hypertrophy and contributes to cardiac dysfunction during pressure overload. (a) Western blotting for CIB1 in the heart of WT, Cib1+/− and Cib1−/− mice. GAPDH is a loading control. (b) Heart weight to body weight ratios (HW/BW) from wildtype (WT), heterozygous or homozygous Cib1 gene-targeted mice 2 weeks after sham or TAC surgery. The number of mice per group is indicated within the bar. *p<0.01 vs. control sham, #p<0.05 vs. WT TAC and Cib1−/− TAC, † p<0.001 vs. WT TAC. (c) Myocyte cross-sectional area in WT and Cib1−/− mice 2 weeks after sham or TAC surgery. *p<0.001 vs. sham, #p<0.001 vs. WT TAC. (d) ANF levels from the indicated hearts after TAC. No expression was observed in sham-operated hearts. *p<0.05 vs. WT TAC. (e) Fractional shortening (FS in %) determined by echocardiography in WT and Cib1−/− mice after sham or TAC surgery. *p<0.01 vs. WT sham and Cib1−/− TAC. N= 6–7 animals per sham group and N=15–18 animals per TAC group. (f) Representative images of Masson’s trichrome-stained paraffin-embedded heart sections from WT and Cib1−/− mice. Scale bars: 100 μm. (g) Myocardial fibrosis as determined by MetaMorph software. N=3 per sham group, N=4 per TAC group, *p<0.01 vs. WT sham, #p<0.01 vs. WT TAC. (h) HW/BW ratio of WT or Cib1−/− mice after 3 weeks of rest or swimming exercise. *p<0.001 vs. WT rest.