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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nat Med. 2010 Jul 18;16(8):872–879. doi: 10.1038/nm.2181

Figure 3.

Figure 3

CIB1 interacts with CnB. (a) β-galactosidase (blue) and growth assessment for protein-protein interaction by yeast two-hybrid analysis. The areas with blue staining represent proteins that are interacting in the assay. (b) Immunoblot for CIB1 (c-myc tag) or CnB after immunoprecipitation (IP) from AdCnB and AdCIB1-myc infected neonatal cardiomyocytes. CnB antibody or control IgG was used for the IP. (c) Immunoblot for CnA or CIB1 after IP from AdCnA and AdCIB1-myc infected neonatal cardiomyocytes. CnA antibody or control IgG was used for the IP. (d) Immunoblot for endogenous CnB or CIB1 after IP from WT mouse hearts. CIB1 antibody or control IgG was used for the IP. (e) GST pull-down assay showing a schematic of the different GST – CIB1 fusion constructs (left). The functional EF-hand domains are marked in green. The right panel shows an SDS PAGE of [35S]methionine-labeled CnB subject to pull-down with the indicated GST-CIB1 fusion constructs. An immunoblot shows the integrity of the GST fusion proteins.