Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nat Med. 2010 Jul 18;16(8):872–879. doi: 10.1038/nm.2181

Figure 6.

Figure 6

Myocardial CIB1 overexpression enhances hypertrophy and calcineurin/NFAT signaling during pressure overload. (a) Schematic representation of the binary transgenic constructs that drive inducible CIB1 expression in the heart. (b) Representative immunoblot for CIB1 and GAPDH (control) from whole cell lysates from hearts of 3 month-old non-transgenic (NTG), single αMHC-tTA (tTA), or αMHCTET-CIB1 × αMHC-tTA double transgenic (DTG) of line (L) 26.6 and 27.5 taken off DOX at 4 weeks of age, or DTG mice from line 27.5 that were never placed on DOX (Always on). (c) HW/BW 2 weeks after sham or TAC surgery from control (Con) and DTG mice of lines 26.6 and 27.5. The number of mice per group is indicated within the bar. *p<0.001 vs. control sham from the same line, #p<0.05 vs. control TAC from the same line. (d) NFAT luciferase activity measured 1 week after sham or TAC surgery in control or DTG mice also harboring the NFAT-luciferase reporter transgene. The number of mice per group is indicated within or above the bar. #p<0.01 vs. control sham and control TAC. (e) Neonatal rat cardiomyocyte average cell size after PE stimulation for 24 hrs and prior infection with Adβgal (control) or AdCIB1. #p<0.01 vs. Adβgal + PE. (f) Representative immunoblot for CnB and GAPDH from whole cell protein lysates from WT control mice and αMHC-ΔCnA-TG mice. (g) HW/BW from control mice, αMHC-ΔCnA-TG mice and αMHC-ΔCnA-TG × αMHCTET-CIB1 × αMHC-tTA triple transgenic (TTG) mice always off DOX. *p<0.001 vs. control, #p<0.001 vs. αMHC-ΔCnA-TG mice. (h) HW/BW of control and line 27.5 DTG mice (always off DOX) 2 weeks after sham or TAC surgery in WT (left panel) or in the Ppp3cb−/− (CnAβ) background (right panel). *p<0.001 vs. control sham, #p<0.05 vs. control TAC.