Dose-dependent cytotoxic response of hepatocellular carcinoma cells to Doxorubicin. PLC (top row) and HepG2 (bottom row) cells were cultured on 2D tissue culture plates, Matrigel matrices, or CA scaffolds for 10 days before treatment with Doxorubicin. Cell viability relative to untreated cells was determined by the Alamar blue assay at 24 h, 48 h and 72 h after Doxorubicin treatment. LD50 was calculated based on viability data. Results are mean ± s.d., and * indicates at least one of the group means is statistically different from the others at that time point, p < 0.05, n = 4.