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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Microbiol. 2010 Jun 11;77(3):618–627. doi: 10.1111/j.1365-2958.2010.07233.x

Figure 1. The electrophoretic mobility of σ80 is greatly reduced during a SpoT-mediated serum starvation response.

Figure 1

Figure 1

Cells of H. pylori wild type strain G27 and its isogenic ΔspoT mutant were grown in BLBB medium, which was either serum-free (A) or supplemented with 10% FBS (B). Cells were sampled at the indicated times and lysed. The σ80 protein in cell lysates was separated by 4–12% SDS-PAGE, followed by protein immunoblotting with the σ80-specific polyclonal antibodies. The positions of σ80 with normal mobility or with reduced electrophoretic mobilities (up-shifted) are indicated. The up-shifted σ80 appeared to be smeared bands, which is likely due to polyP being heterogeneous in size (see text).