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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Mol Microbiol. 2010 Jun 11;77(3):618–627. doi: 10.1111/j.1365-2958.2010.07233.x

Figure 4. Mutational analysis of the determinants during serum starvation response.

Figure 4

Figure 4

(A) Both the σ80Δ56 and ΔppK mutants were defective in forming theσ80·polyP complexes during serum starvation. σ80 was un-shifted in both the σ80Δ56 and ΔppK mutant cells grown serum-free. Cells were analyzed as described in the legend of Figure 1. (B). Both the σ80Δ56 and ΔppK mutants exhibited reduced survival during serum starvation, approaching the premature death phenotype of the ΔspoT mutant at day 5. The numbers of viable bacteria are expressed as colony-forming unit (CFU). The error bars indicate the deviations from the means, and there are no error bars indicated when the deviations were smaller than the symbol. The data are representative of three independent experiments.