Fig. 2.

Effect of rose absolute oil (RAO) on involucrin expression in keratinocytes. (A) Normal human epidermal keratinocytes were treated with RAO at the indicated concentrations for 3 days. Calcium (1.2 mM) was included as a positive control for keratinocyte differentiation. Cellular proteins were prepared and protein level for involucrin was evaluated by Western blot. Actin was used as a loading control. (B) Effect of RAO on the involucrin promoter activity. Keratinocytes were transduced with 10 multiplicity of infections (MOIs) of involucrin-luc reporter adenovirus, then treated with RAO for 3 days. Cells were lysed and assayed for luciferase activity. Data are represented as relative light unit (RLU) and SEM, measured from 3 independent experiments.