Fig. 5.

Effect of rose absolute oil (RAO) on transepidermal water loss (TEWL). (A) Hairless mice were tape-stripped, then topically treated with 0.1% solution of RAO (in 70% polyethylene glycol and 30% ethanol) and RAO complex in which RAO (0.1%), oleic acid (0.1%), oleanolic acid (0.05%), urocanic acid (0.01%), acetylhexapeptide (0.01%) and ceramide (0.1%) were additionally included. At the indicated time points, TEWL was measured using Tewameter. Basal TEWL (0 h) was measured before tape-stripping. Five hairless mice were included in each group. Data are represented as average and SEM. (B) Skin specimens were obtained after 5 days of treatment. Sections were prepared and stained with anti-filaggrin antibody (×100).