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Contact allergen-DNCB, but not irritant-BKC, induced protein carbonylation. Immature day 6 MoDCs were incubated with 20µm DNCB and 10µM BKC for 6 h. Carbonylated groups were detected by immunoblotting with an anti-DNP antibody. DNCB treated cells but not BKC treated cells showed increased carbonylation compared with the control. Actin is included as a loading control. These data are representative of two independent experiments.
