Fig. 3.

Augmentation of CD86 and HLA-DR expression on MoDCs by DNCB but not by BKC. MoDCs were treated with 10µM DNCB or 5µM BKC for 24 h and then analyzed by flow cytometry. (A, B) Thick solid line indicates CD86 expression on MoDCs after treatment with DNCB and BKC, respectively; solid narrow line indicates positive control (treatment with cytokine cocktail); filled line indicates untreated DCs; dotted line indicates DCs stained with CD86 only. (C, D) Thick solid line indicates HLA-DR expression on MoDCs after treatment with DNCB and BKC, respectively; solid narrow line indicates positive control, filled line indicates untreated DCs, and dotted line indicates DCs stained with HLA-DR only. CD86 and HLA-DR expression on MoDCs cultured with DNCB was increased 2.5-fold and 1.2-fold respectively; p-values were 0.018 and 0.033, respectively, compared to that of the control.