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. Author manuscript; available in PMC: 2011 Jan 23.
Published in final edited form as: Circ Res. 2010 May 27;107(2):283–293. doi: 10.1161/CIRCRESAHA.110.221663

Figure 7. Dll4 inhibition increases the release of CXCL1/IL-8.

Figure 7

A, Ad-sDll4 increases the circulating levels of CXCL1 in mice with limb ischemia. *P<0.05, **P*lt;0.001 vs Ad-Null; #P<0.05, **P<0.01 vs nonischemic mice (control); n=6 each group. B, Left, IL-8 concentrations in conditioned media of THP1 monocytes cultured on immobilized Dll4 in the presence of TNF-α or vehicle. To block Notch, DAPT or vehicle (DMSO) was added. Right, Hes1 expression levels in the same experiment. *P<0.001 vs BSA; #P<0.001 vs vehicle; n=4. n.d. indicates not detectable. C, Left, IL-8 concentrations in conditioned media of THP1 monocytes cocultured with transfected HUVECs. Right, Hes1 expression levels in the same experiment. *P<0.001 vs BSA; #P<0.01 vs Ad-Null; n=4. D, Time-lapse video microscopy images illustrating the endothelial network formation by HUVECs on Matrigel in the presence of DiI-labeled THP1 cells (red). HUVECs were transfected with Ad-Null or Ad-sDll4 (100 plaque-forming units/cell).