Skip to main content
. Author manuscript; available in PMC: 2011 Aug 6.
Published in final edited form as: Sens Actuators B Chem. 2010 Aug 6;149(1):325–328. doi: 10.1016/j.snb.2010.06.024

Figure 3.

Figure 3

Demonstration of signal amplification using a simple 3 inlet 2DPN. A spot of BSA-biotin was located upstream of the wicking pad and reagents were applied approximately simultaneously to the source pads using a multi-channel pipette in a single activation step. The images correspond to the approximate times of 1 s, 5 s, 25 s, 2 min, 5.5 min, and 20 min for t1 through t6, respectively. Streptavidin labeled gold (SA-G) reached the capture spot first, next a phosphate buffered saline (PBS) wash, and finally a gold enhancement (GE) solution. Note that the capture spot to the left of the wicking pad becomes substantially darker by t6.