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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nat Struct Mol Biol. 2010 Jul 11;17(8):939–947. doi: 10.1038/nsmb.1873

Figure 6.

Figure 6

Hydrolysis of Lys11 linkages by DUBs.

(a, b) Analysis of a panel of DUBs from all human DUB families against Lys11- (a) and Lys63-linked (b) diubiquitin. Two lanes per DUB in a silver stained gel indicate hydrolysis of ubiquitin dimers after 10 and 60 min, respectively. The Lys63-linked dimers were not tested against UCH family members as these were previously found to be inactive against this chain type 33. (c) The panel of DUBs was analyzed against Lys11-polyubiquitinated UBE2S and ubiquitin was visualized by Western blotting.