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. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Nat Struct Mol Biol. 2010 Jul 11;17(8):939–947. doi: 10.1038/nsmb.1873

Figure 7.

Figure 7

Cezanne cleaves Lys11-linkages preferentially

(a) Phylogenetic tree of the OTU family DUBs 41, showing the close relationship of A20, TRABID and Cezanne. (b) Schematic presentation of domain structures of the OTU family DUBs Cezanne, A20 and TRABID. (c-e) Specificity analysis of Cezanne (c), A20 (d) and TRABID (e) against Lys11-, Lys48-, Lys63- and linear ubiquitin tetramers, resolved on 4-12% SDS-PAGE gels and visualized by silver staining. In this comparison, Cezanne shows specificity for Lys11-linked chains, while A20 and TRABID remain Lys48- and Lys63-specific, respectively. (f) Cezanne prefers Lys11-linkages in a mixture of differently linked ubiquitin tetramers. The different electrophoretic mobility of the individual tetraubiquitin molecules allows identification of the linkage type.