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. Author manuscript; available in PMC: 2011 Jul 8.
Published in final edited form as: J Med Chem. 2010 Jul 8;53(13):5033–5043. doi: 10.1021/jm100274c

Figure 1.

Figure 1

Effects of resveratrol and its sulfate derivatives on iNOS and COX-2 expression in LPS-stimulated RAW 264.7 cells. RAW 264.7 cells were treated with compounds 15 min prior to LPS (1 μg/mL) stimulation and further incubated for 18 h. Cells were lysed, and protein (30 μg) was subjected to 8% SDS-PAGE. The level of iNOS and COX-2 protein expression was examined by immunoblot analysis. β-Actin was used as an internal control. The experiment was performed in duplicate. The density of each band was measured by using an image analyzer system. Average values of relative iNOS protein expression are shown in comparison with the LPS-treated control (black bar). Compounds 1, 5 and 7 (open bars) showed suppressive effects on the expression of iNOS protein with relative values of 0.62, 0.54 and 0.67, respectively. Compounds 2, 3, and 4 were not active, and COX-2 expression was not altered.