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Schematic depiction of the lacZ-plasmid construct as it is integrated at loci on Drosophila chromosomes 2L and 3R. LacZ-plasmids were excised at HindIII sites, separated from the fly chromosomal DNA, circularized by ligation and used to transform E. coli ΔlacZ, galE− as described (Garcia et al. 2007a). LacZ mutants are positively selected on p-gal (red colonies) and the titer determined by plating a small fraction of the transformants on X-gal (blue colonies).
