Fig. 1.

α-1A-Adrenergic augmentation of HERG protein. A, immunoblots from clonal HEK cell lines stably expressing both HERG and the α-1A-adrenergic receptor with and without 24-h phenylephrine treatment with tubulin loading control and immunoblots for the α-1A-adrenergic receptor. B, the specific α-1A-adrenergic receptor antagonist 5-MU blocked the phenylephrine-dependent augmentation of HERG protein (histogram provides summary data for different treatment groups with separate bars for the immature 135- and mature 155-kDa forms). C, clone 1 from A treated with differing concentrations of phenylephrine applied for 24 h. Bottom, tubulin loading control. Histogram shows the average increase in HERG. D, clone 1 from A treated with 1 μM PE for varying durations. Summary data are from four to five separate experiments; ∗, significantly different from control values with p < 0.05.