Fig. 3.

PKC involvement in α1A-adrenergic augmentation of HERG channel protein. A, immunoblots from a clonal cell line stably expressing HERG and α1A-AR. PE treatment for 24 h increases HERG abundance, an effect that is reduced by Ro 32-0432 (Ro-32) and abolished by Bis-I when PE concentration is 0.1 μM. When the PE concentration is increased to 1 μM, both PKC inhibitors only partially prevent the augmentation of HERG (right histogram shows densitometry results; ∗, p < 0.02; ∗∗, p < 0.002, n = 3.). B, similar conditions as in A showing inhibition of the receptor-mediated augmentation of HERG by another PKC inhibitor, Gö6976. A similar pattern of incomplete inhibition is seen when receptors are maximally stimulated.