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. 2010 Aug;78(2):166–174. doi: 10.1124/mol.110.065011

Fig. 2.

Fig. 2.

GO-201 blocks nuclear targeting of NF-κB p65 and induces late apoptosis/necrosis. A and B, U266 (A) and RPMI8226 (B) cells were left untreated (Control) and treated with 5 μM GO-201 or CP-1 each day for 2 days. Nuclear lysates were immunoblotted with the indicated antibodies. C and D, U266 (C) and RPMI8226 (D) cells were left untreated (CTL) and treated with 5 μM GO-201 or CP-1 each day for 3 days. Cells were stained with PI/annexin V and analyzed by flow cytometry (left). The percentage of cells positive for both PI and annexin V is indicated in the top right quadrants. The results are expressed as the percentage (mean ± S.D. of three determinations) of late apoptotic/necrotic cells (right).