Fig. 4.

GO-203 inhibits constitutive activation of the NF-κB p65 pathway. U266 (left) and RPMI8226 (right) cells were left untreated (Control) and treated with 5 μM GO-203 or CP-2 each day for 2 days. A, anti-MUC1-C precipitates were immunoblotted with anti-p65 and anti-MUC1-C. B, nuclear lysates were immunoblotted with the indicated antibodies. C, soluble chromatin was precipitated with anti-p65. The final DNA extractions were amplified by polymerase chain reaction with pairs of primers that cover the NF-κB response element (−597 to −304) in the Bcl-xL promoter. D, Whole-cell lysates were immunoblotted with the indicated antibodies.