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Endogenous interaction of spinophilin with the α2AAR in mouse brain. HA-α2AAR knock-in mice were intraperitoneally injected with saline (control) or UK14,304 (1 mg/kg). Thirty minutes after injection, mouse brains were isolated, homogenized, and coimmunoisolation assays of detergent-solubilized preparations were performed as described under Materials and Methods. Shown are representative blots from three or more independent experiments.
