Table 2.
Loss of memory (A) CD8+ T-cell number (× 104) and (B) percentage in spleen after heterologous viral infection
| (A) | VV B8R 20-27-specific | LCMV GP 33/34-41-specific | LCMV NP 396-404-specific | |||
|---|---|---|---|---|---|---|
| VV | VV+LCMV | LCMV | LCMV+MCMV | LCMV | LCMV+MCMV | |
| 27 | 16 | 23 | 7.4 | 13 | 4.5 | |
| 32 | 10 | 19 | 9.1 | 17 | 6.8 | |
| 8.4 | 12 | 19 | 9.5 | 11 | 8.6 | |
| 24 | 3.3 | 12 | 17 | 5.8 | 7.8 | |
| 35 | 3.8 | 33 | 9.1 | 19 | 6.3 | |
| mean | 25 | 9.0 | 21 | 10 | 13 | 6.8 |
| ↓64% (p = 0.01) | ↓51% (p = 0.02) | ↓ 48% (p = 0.03) | ||||
| (B) | VV B8R 20-27-specific | LCMV GP33/34-specific | LCMV NP 396-404-specific | |||
|---|---|---|---|---|---|---|
| VV | VV+LCMV | LCMV | LCMV+MCMV | LCMV | LCMV+MCMV | |
| 2.2% | 1.2% | 3.1% | 0.84% | 1.9% | 0.51% | |
| 3.0% | 0.87% | 2.7% | 0.73% | 2.3% | 0.55% | |
| 0.96% | 0.94% | 1.9% | 1.3% | 1.1% | 1.2% | |
| 2.3% | 0.42% | 1.9% | 2.4% | 0.95% | 1.1% | |
| 2.7% | 0.38% | 3.0% | 0.92% | 1.7% | 0.64% | |
| mean | 2.2% | 0.76% | 2.5% | 1.2% | 1.6% | 0.80% |
| ↓66% (p = 0.005) | ↓51% (p = 0.01) | ↓50% (p = 0.03) | ||||
C57BL/6 mice were infected with sublethal doses of VV, strain WR, or LCMV, strain Armstrong, and allowed to clear virus and enter a resting memory state. The VV-immune mice were then challenged with LCMV or vehicle, and the LCMV-immune mice were challenged with MCMV or vehicle. The numbers represent the total numbers (top) or frequencies per CD8 T cell (bottom) of some of their immundominant epitopes per spleen. GP33/34 represents the combined response of overlapping Db- and Kb-restricted epitopes. Values given are for 5 individual mice per group and their means and p values calculated by Student’s t-test.